Chromatin Immunoprecipitation:Article Title: DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Rabbit anti-FANCD2 Novus CatNB100-182, RRID: AB_1108498 Mouse anti-FANCD2 Santa Cruz Cat#sc-20022, RRID: AB_2278211 Mouse anti-53BP1(6B3E10) Santa Cruz Cat#sc-517281, RRID:AB_2921289 Rabbit anti-RPA, Made in-house PMID: 32075739 Mouse anti-α-Tubulin Sigma-Aldrich Cat# T9026, RRID:AB_477593 Rabbit anti-GAPDH Sigma-Aldrich Cat#PLA0125, RRID:AB_2910561 Mouse anti-Histone H3 Abcam Cat# ab1791, RRID:AB_302613 Rabbit anti-Histone H3,phospho (Ser10) Abcam Cat# ab5176, RRID:AB_304763 Rabbit anti-gammaH2AX Millipore Catalog # 05-636, RRID:AB_309864 Rabbit anti-Human POLD3 Abcam Cat# ab182564 Rabbit anti-BLM, Abcam Cat #A300-110A Rabbit anti-BRCA1, Abcam, Cat#ab19042 Mouse anti-BRCA1 Abcam Cat #AB16780 1 Rabbit anti-CtIP, Abcam, Cat#ab155988 Rabbit anti-DNA2, Invitrogen, Cat#PA5-68167, AB_2691658 Rabbit anti-EXO1, Abcam, Cat#ab95012 Rabbit anti-MRE11a, Novus Biologicals, Cat#NB100-142, RRID:AB_350079 Mouse anti-MUS81, Santa Cruz, Cat#sc53382, RRID:AB_2147138 Rabbit anti-Myc-tag, Abcam, Cat#ab9106 Mouse anti-NBS1, Abcam, Cat#ab181729 Rabbit anti-pCtIP-Thr847, Abcam, Cat#ab254068 4 nature research | reporting sum m ary O ctober 2018 Mouse anti-RAD50, Abcam, Cat#ab89, RRID:AB_2176935 RAbbit anti-RAD51 BioAcademia CAt#70-002 Rabbit anti-RAD52, Abcam, Cat#ab103067 Mouse anti-WRN, Abcam, Cat#ab241545 Mouse anti-RPA32 Santa Cruz Cat##sc-56770 Anti-mouse IgG, HRP-linked Antibody Sigma-Aldrich Cat# A4416, RRID:AB_258167 Anti-rabbit IgG, HRP-linked Antibody Sigma-Aldrich Cat# A6667, RRID:AB_258307 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 ThermoFisher Scientific Cat# A-11029, RRID:AB_2534088 Goat anti-Rabbit IgG (H+L) Highly Cross-adsorbed Antibody, Alexa Fluor 568 Conjugated ThermoFisher Scientific Cat# A-11036, RRID:AB_10563566 Validation Most of the antibodies used in this study have been validated by previous studies as shown in the references in the Methods. ..
Flow Cytometry:Article Title: DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Rabbit anti-FANCD2 Novus CatNB100-182, RRID: AB_1108498 Mouse anti-FANCD2 Santa Cruz Cat#sc-20022, RRID: AB_2278211 Mouse anti-53BP1(6B3E10) Santa Cruz Cat#sc-517281, RRID:AB_2921289 Rabbit anti-RPA, Made in-house PMID: 32075739 Mouse anti-α-Tubulin Sigma-Aldrich Cat# T9026, RRID:AB_477593 Rabbit anti-GAPDH Sigma-Aldrich Cat#PLA0125, RRID:AB_2910561 Mouse anti-Histone H3 Abcam Cat# ab1791, RRID:AB_302613 Rabbit anti-Histone H3,phospho (Ser10) Abcam Cat# ab5176, RRID:AB_304763 Rabbit anti-gammaH2AX Millipore Catalog # 05-636, RRID:AB_309864 Rabbit anti-Human POLD3 Abcam Cat# ab182564 Rabbit anti-BLM, Abcam Cat #A300-110A Rabbit anti-BRCA1, Abcam, Cat#ab19042 Mouse anti-BRCA1 Abcam Cat #AB16780 1 Rabbit anti-CtIP, Abcam, Cat#ab155988 Rabbit anti-DNA2, Invitrogen, Cat#PA5-68167, AB_2691658 Rabbit anti-EXO1, Abcam, Cat#ab95012 Rabbit anti-MRE11a, Novus Biologicals, Cat#NB100-142, RRID:AB_350079 Mouse anti-MUS81, Santa Cruz, Cat#sc53382, RRID:AB_2147138 Rabbit anti-Myc-tag, Abcam, Cat#ab9106 Mouse anti-NBS1, Abcam, Cat#ab181729 Rabbit anti-pCtIP-Thr847, Abcam, Cat#ab254068 4 nature research | reporting sum m ary O ctober 2018 Mouse anti-RAD50, Abcam, Cat#ab89, RRID:AB_2176935 RAbbit anti-RAD51 BioAcademia CAt#70-002 Rabbit anti-RAD52, Abcam, Cat#ab103067 Mouse anti-WRN, Abcam, Cat#ab241545 Mouse anti-RPA32 Santa Cruz Cat##sc-56770 Anti-mouse IgG, HRP-linked Antibody Sigma-Aldrich Cat# A4416, RRID:AB_258167 Anti-rabbit IgG, HRP-linked Antibody Sigma-Aldrich Cat# A6667, RRID:AB_258307 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 ThermoFisher Scientific Cat# A-11029, RRID:AB_2534088 Goat anti-Rabbit IgG (H+L) Highly Cross-adsorbed Antibody, Alexa Fluor 568 Conjugated ThermoFisher Scientific Cat# A-11036, RRID:AB_10563566 Validation Most of the antibodies used in this study have been validated by previous studies as shown in the references in the Methods. ..
Magnetic Resonance Imaging:Article Title: DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Rabbit anti-FANCD2 Novus CatNB100-182, RRID: AB_1108498 Mouse anti-FANCD2 Santa Cruz Cat#sc-20022, RRID: AB_2278211 Mouse anti-53BP1(6B3E10) Santa Cruz Cat#sc-517281, RRID:AB_2921289 Rabbit anti-RPA, Made in-house PMID: 32075739 Mouse anti-α-Tubulin Sigma-Aldrich Cat# T9026, RRID:AB_477593 Rabbit anti-GAPDH Sigma-Aldrich Cat#PLA0125, RRID:AB_2910561 Mouse anti-Histone H3 Abcam Cat# ab1791, RRID:AB_302613 Rabbit anti-Histone H3,phospho (Ser10) Abcam Cat# ab5176, RRID:AB_304763 Rabbit anti-gammaH2AX Millipore Catalog # 05-636, RRID:AB_309864 Rabbit anti-Human POLD3 Abcam Cat# ab182564 Rabbit anti-BLM, Abcam Cat #A300-110A Rabbit anti-BRCA1, Abcam, Cat#ab19042 Mouse anti-BRCA1 Abcam Cat #AB16780 1 Rabbit anti-CtIP, Abcam, Cat#ab155988 Rabbit anti-DNA2, Invitrogen, Cat#PA5-68167, AB_2691658 Rabbit anti-EXO1, Abcam, Cat#ab95012 Rabbit anti-MRE11a, Novus Biologicals, Cat#NB100-142, RRID:AB_350079 Mouse anti-MUS81, Santa Cruz, Cat#sc53382, RRID:AB_2147138 Rabbit anti-Myc-tag, Abcam, Cat#ab9106 Mouse anti-NBS1, Abcam, Cat#ab181729 Rabbit anti-pCtIP-Thr847, Abcam, Cat#ab254068 4 nature research | reporting sum m ary O ctober 2018 Mouse anti-RAD50, Abcam, Cat#ab89, RRID:AB_2176935 RAbbit anti-RAD51 BioAcademia CAt#70-002 Rabbit anti-RAD52, Abcam, Cat#ab103067 Mouse anti-WRN, Abcam, Cat#ab241545 Mouse anti-RPA32 Santa Cruz Cat##sc-56770 Anti-mouse IgG, HRP-linked Antibody Sigma-Aldrich Cat# A4416, RRID:AB_258167 Anti-rabbit IgG, HRP-linked Antibody Sigma-Aldrich Cat# A6667, RRID:AB_258307 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 ThermoFisher Scientific Cat# A-11029, RRID:AB_2534088 Goat anti-Rabbit IgG (H+L) Highly Cross-adsorbed Antibody, Alexa Fluor 568 Conjugated ThermoFisher Scientific Cat# A-11036, RRID:AB_10563566 Validation Most of the antibodies used in this study have been validated by previous studies as shown in the references in the Methods. ..
Biomarker Discovery:Article Title: DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Rabbit anti-FANCD2 Novus CatNB100-182, RRID: AB_1108498 Mouse anti-FANCD2 Santa Cruz Cat#sc-20022, RRID: AB_2278211 Mouse anti-53BP1(6B3E10) Santa Cruz Cat#sc-517281, RRID:AB_2921289 Rabbit anti-RPA, Made in-house PMID: 32075739 Mouse anti-α-Tubulin Sigma-Aldrich Cat# T9026, RRID:AB_477593 Rabbit anti-GAPDH Sigma-Aldrich Cat#PLA0125, RRID:AB_2910561 Mouse anti-Histone H3 Abcam Cat# ab1791, RRID:AB_302613 Rabbit anti-Histone H3,phospho (Ser10) Abcam Cat# ab5176, RRID:AB_304763 Rabbit anti-gammaH2AX Millipore Catalog # 05-636, RRID:AB_309864 Rabbit anti-Human POLD3 Abcam Cat# ab182564 Rabbit anti-BLM, Abcam Cat #A300-110A Rabbit anti-BRCA1, Abcam, Cat#ab19042 Mouse anti-BRCA1 Abcam Cat #AB16780 1 Rabbit anti-CtIP, Abcam, Cat#ab155988 Rabbit anti-DNA2, Invitrogen, Cat#PA5-68167, AB_2691658 Rabbit anti-EXO1, Abcam, Cat#ab95012 Rabbit anti-MRE11a, Novus Biologicals, Cat#NB100-142, RRID:AB_350079 Mouse anti-MUS81, Santa Cruz, Cat#sc53382, RRID:AB_2147138 Rabbit anti-Myc-tag, Abcam, Cat#ab9106 Mouse anti-NBS1, Abcam, Cat#ab181729 Rabbit anti-pCtIP-Thr847, Abcam, Cat#ab254068 4 nature research | reporting sum m ary O ctober 2018 Mouse anti-RAD50, Abcam, Cat#ab89, RRID:AB_2176935 RAbbit anti-RAD51 BioAcademia CAt#70-002 Rabbit anti-RAD52, Abcam, Cat#ab103067 Mouse anti-WRN, Abcam, Cat#ab241545 Mouse anti-RPA32 Santa Cruz Cat##sc-56770 Anti-mouse IgG, HRP-linked Antibody Sigma-Aldrich Cat# A4416, RRID:AB_258167 Anti-rabbit IgG, HRP-linked Antibody Sigma-Aldrich Cat# A6667, RRID:AB_258307 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 ThermoFisher Scientific Cat# A-11029, RRID:AB_2534088 Goat anti-Rabbit IgG (H+L) Highly Cross-adsorbed Antibody, Alexa Fluor 568 Conjugated ThermoFisher Scientific Cat# A-11036, RRID:AB_10563566 Validation Most of the antibodies used in this study have been validated by previous studies as shown in the references in the Methods. ..
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